全文获取类型
收费全文 | 703篇 |
免费 | 34篇 |
出版年
2023年 | 2篇 |
2022年 | 1篇 |
2021年 | 9篇 |
2020年 | 2篇 |
2019年 | 10篇 |
2018年 | 5篇 |
2017年 | 7篇 |
2016年 | 17篇 |
2015年 | 26篇 |
2014年 | 26篇 |
2013年 | 37篇 |
2012年 | 55篇 |
2011年 | 55篇 |
2010年 | 29篇 |
2009年 | 35篇 |
2008年 | 44篇 |
2007年 | 49篇 |
2006年 | 47篇 |
2005年 | 40篇 |
2004年 | 53篇 |
2003年 | 29篇 |
2002年 | 37篇 |
2001年 | 10篇 |
2000年 | 8篇 |
1999年 | 13篇 |
1998年 | 7篇 |
1997年 | 4篇 |
1996年 | 8篇 |
1995年 | 10篇 |
1994年 | 8篇 |
1993年 | 9篇 |
1992年 | 12篇 |
1991年 | 10篇 |
1990年 | 3篇 |
1989年 | 6篇 |
1988年 | 2篇 |
1985年 | 1篇 |
1984年 | 3篇 |
1983年 | 2篇 |
1981年 | 2篇 |
1978年 | 1篇 |
1974年 | 1篇 |
1973年 | 1篇 |
1972年 | 1篇 |
排序方式: 共有737条查询结果,搜索用时 15 毫秒
61.
Kojima K Oshita M Nanjo Y Kasai K Tozawa Y Hayashi H Nishiyama Y 《Molecular microbiology》2007,65(4):936-947
Oxidative stress inhibits the repair of photodamaged photosystem II (PSII). This inhibition is due initially to the suppression, by reactive oxygen species (ROS), of the synthesis de novo of proteins that are required for the repair of PSII, such as the D1 protein, at the level of translational elongation. To investigate in vitro the mechanisms whereby ROS inhibit translational elongation, we developed a translation system in vitro from the cyanobacterium Synechocystis sp. PCC 6803. The synthesis of the D1 protein in vitro was inhibited by exogenous H2O2. However, the addition of reduced forms of elongation factor G (EF-G), which is known to be particularly sensitive to oxidation, was able to reverse the inhibition of translation. By contrast, the oxidized forms of EF-G failed to restore translational activity. Furthermore, the overexpression of EF-G of Synechocystis in another cyanobacterium Synechococcus sp. PCC 7942 increased the tolerance of cells to H2O2 in terms of protein synthesis. These observations suggest that EF-G might be the primary target, within the translational machinery, of inhibition by ROS. 相似文献
62.
Three strands of 30-mer oligodeoxyribonucleotides (ODNs) were designed to form three-way junctions that possess self-complementary sticky ends. The morphology of self-assembled ODNs in water was observed in situ by confocal laser scanning fluorescence microscopy. The three-way junctions self-assembled into spherical assemblies, in accordance with transmission and scanning electron microscopy. The size of nucleospheres was in the range of several tens of nanometers to micrometers, which varied depending on the concentration of ODNs and added salts. Fluorescence images of spherical ODN assemblies suggested that the nucleospheres possess multiwalled structures. The fluorescence of sodium 1-anilinonaphthalene-8-sulfonate in the presence of nucleospheres revealed that the interior of nucleospheres possesses polarity corresponding to that between methanol and ethanol. A dye-inclusion experiment showed that cationic and even anionic dyes were adsorbed to the interior of the nucleospheres. The dye-included nucleospheres released dyes by thermal dissociation or digestion of the constituent ODNs. 相似文献
63.
Ujifuku Kenta Fujimoto Takashi Sato Kei Morofuji Yoichi Muto Hideki Masumoto Hiroshi Nakagawa Shinsuke Niwa Masami Matsuo Takayuki 《Cellular and molecular neurobiology》2022,42(4):997-1004
Cellular and Molecular Neurobiology - Metastatic brain tumors have poor prognoses and pose unmet clinical problems for the patients. The blood–brain barrier (BBB) implication is supposed to... 相似文献
64.
65.
Inhibition of RANKL‐dependent cellular fusion in pre‐osteoclasts by amiloride and a NHE10‐specific monoclonal antibody 下载免费PDF全文
66.
67.
Tetsuji Nakabo Kouji Nakayama Nozomu Muto Masayuki Miyazawa 《Ichthyological Research》2011,58(2):180-183
Oncorhynchus kawamurae (Osteichthyes: Salmonidae) (common name “Kunimasu”), a species endemic to Lake Tazawa, Akita Prefecture, Japan, was believed
to have been extinct since 1940. However, nine specimens were discovered in March and April 2010 in Lake Saiko, Yamanashi
Prefecture, one of the lakes to which eyed eggs of the species were introduced in 1935. These were identified as O. kawamurae because of having 47–62 pyloric caeca, 37–43 gill-rakers, a black-colored body, and spawning at 30–40 m depth in early spring,
which are unique characteristics within Oncorhynchus. Furthermore, the distinctiveness of Kunimasu from sympatric kokanee (O. nerka) was supported by microsatellite DNA data. 相似文献
68.
Takeshi Kikko Yoshitaka Kataoka Katsuhiro Nishimori Yasuhiro Fujioka Yoshiaki Kai Kouji Nakayama Toshihide Kitakado 《Ichthyological Research》2011,58(4):370-376
Size at maturity of fluvial white-spotted charr, Salvelinus leucomaenis, was studied in small headwater tributaries of nine rivers around the Lake Biwa water system, Japan. Threshold size at maturity
in both sexes showed significant positive relationships with water discharge, indicating that smaller threshold sizes at maturity
of fluvial white-spotted charr occurred in smaller habitats. These results provide a link between size at maturity and habitat
size and have important implications for the management of both habitats and white-spotted charr populations. 相似文献
69.
70.
Kawamoto T Araki K Sonoda E Yamashita YM Harada K Kikuchi K Masutani C Hanaoka F Nozaki K Hashimoto N Takeda S 《Molecular cell》2005,20(5):793-799
Chicken B lymphocyte precursors and DT40 cells diversify their immunoglobulin-variable (IgV) genes through homologous recombination (HR)-mediated Ig gene conversion. To identify DNA polymerases that are involved in Ig gene conversion, we created DT40 clones deficient in DNA polymerase eta (poleta), which, in humans, is defective in the variant form of xeroderma pigmentosum (XP-V). Poleta is an error-prone translesion DNA synthesis polymerase that can bypass UV damage-induced lesions and is involved in IgV hypermutation. Like XP-V cells, poleta-disrupted (poleta) clones exhibited hypersensitivity to UV. Remarkably, poleta cells showed a significant decrease in the frequency of both Ig gene conversion and double-strand break-induced HR when compared to wild-type cells, and these defects were reversed by complementation with human poleta. Our findings identify a DNA polymerase that carries out DNA synthesis for physiological HR and provides evidence that a single DNA polymerase can play multiple cellular roles. 相似文献